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Transplantations with bone marrow or stem cells obtained from related donors have been widely accepted in the treatment of many hematological diseases. However, the success of BMT is still severely limited by the relatively high incidence of graft versus host disease and by the lack of suitable donors. It appears that patient selection is a key determinant of the success of BMT. This selection includes the selection of healthy donors, the evaluation of the patients before stem cell mobilization, and the selection of patients with a favorable disease. Knowledge of hematological diseases and appropriate management can aid in patient selection. As most of hematological diseases are chronic disorders, the potential for cure is greatly increased by appropriate management, which includes the follow-up of the patients. We believe that a better understanding of the disease will lead to improvements in patient selection and treatment protocols, which will result in increasing the survival of patients with hematological malignancies.Comparison of the biological behaviors of the human monocytoid cell line THP-1 with human peripheral blood monocytes and human monocyte-derived macrophages.
The THP-1 human cell line was derived from a patient with acute myelocytic leukemia. THP-1 is a homogeneous population of cells with adhesion characteristics similar to monocytes, and the cell line can differentiate to both monocyte- and macrophage-like cells. The cell line has proven to be an invaluable tool for the study of monocytes and macrophages. The literature has shown that THP-1 has similar biochemical characteristics to blood-derived monocytes and macrophages, with some differences. In the present study, we examined the biological behavior of THP-1 with a series of assays designed to compare with human blood monocytes and macrophages. The results indicated that the THP-1 cells have many of the biochemical
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