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Trout gonadotrophs are widely used for the study of the neuroendocrine regulation of sexual development, reproductive physiology and the conservation of this important vertebrate model. The authors present here a protocol for cryopreservation of these cells, as well as for long-term storage of trout pituitary secretions. The cells are cryopreserved by slow freezing in 10% dimethyl sulfoxide (DMSO) at -80 degrees C. Prior to the freezing step, the cells are incubated for 2 h in a medium free of DMSO. The re-suspended cells are then transferred to a 1.8-ml Eppendorf tube containing the same freezing medium and a 30-50% layer of glycerol at -20 degrees C. An aliquot of cells are thawed for direct cell counting and the supernatant is frozen at -70 degrees C. Viability is greater than 90% in cells thawed immediately or after 24-48 h. The freezing step reduces gonadotroph viability to 80%.Q:
Matching GPS Coordinates In SQL Server 2008 R2
I have the following format to capture GPS Coordinates:
Lat [decimal Degrees] Long [decimal Degrees] (e.g. 39.76 1.34)
Then I have another table with the actual GPS coordinates:
latitude [decimal Degrees] latitude [decimal Degrees] longitude [decimal Degrees] longitude [decimal Degrees]
What I would like to do is query the coordinate table and if a GPS coordinates matches and also has the same latitude and longitude value, then do a SELECT on the original table with the GPS coordinates.
Does anyone have any ideas on how I could write this? Is there a better way? I’ve been searching but I cannot figure out how to even start to write this…
The data does not need to be exact, as long as the latitude and longitude match. Any help would be greatly appreciated! Thanks!
A:
If lat and lon are always going to be the same then you can use a WHERE clause to compare:
SELECT *
FROM
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